RS/tRNA Foundational Publication Support
Lang, Kathrin, Lloyd Davis, Stephen Wallace, Mohan Mahesh, Daniel J Cox, Melissa L Blackman, Joseph M Fox, and Jason W Chin. (2012) 2012. “Genetic Encoding Of Bicyclononynes And Trans-Cyclooctenes For Site-Specific Protein Labeling In Vitro And In Live Mammalian Cells Via Rapid Fluorogenic Diels-Alder Reactions.”. Journal Of The American Chemical Society 134 (25): 10317-20. doi:10.1021/ja302832g.
RS/tRNA Pair Development Year
2012
RS Organism of Origin
Parent RS
RS Mutations
Y271A
L274M
C313A
L274M
C313A
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
n/a
RS/tRNA Additional Notes
TCORS enables amino acid dependent synthesis of sfGFP from psf GFP150TAGPylT-His6 in the presence of sTCO, confirmed by ESI-MS.
Western blotting confirmed efficient incorporation of TCO into mammalian cells using TCORS
Western blotting confirmed efficient incorporation of TCO into mammalian cells using TCORS