RS/tRNA Foundational Publication Support
Stokes, Audrey L, Shigeki J Miyake-Stoner, Jennifer C Peeler, Duy P Nguyen, Robert P Hammer, and Ryan A Mehl. (2009) 2009. “Enhancing The Utility Of Unnatural Amino Acid Synthetases By Manipulating Broad Substrate Specificity.”. Molecular Biosystems 5 (9): 1032-8. doi:10.1039/b904032c.
RS/tRNA Pair Development Year
2009
ncAA(s) Incorporated
4-Fluoro-p-benzoyl-L-phenylalanine(4F-Bpa)
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Demonstration that one synthetase can have a rationally designed broad scope
Thyronine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
n/a
p-benzoyl-l-phenylalanine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Used as a photocrosslinker, allowing for crosslinking and bioorthogonal ligation of protein.
RS Organism of Origin
Parent RS
RS Mutations
T158S
A31V
A31V
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
In DH10B cells, media containing 1mM 4-Fluoro-p-benzoyl-L-phenylalanine(4F-Bpa) resulted in Bpa-RS-T158S,A31V yielding approximately 1000 mg/L sfGFP.
In DH10B cells, media containing 1mM Thyronine resulted in Bpa-RS-T158S,A31V yielding just over 1000 mg/L sfGFP.
In DH10B cells, media containing 1mM p-benzoyl-l-phenylalanine resulted in Bpa-RS-T158S,A31V yielding just over 850 mg/L sfGFP.
In DH10B cells, media containing 1mM Thyronine resulted in Bpa-RS-T158S,A31V yielding just over 1000 mg/L sfGFP.
In DH10B cells, media containing 1mM p-benzoyl-l-phenylalanine resulted in Bpa-RS-T158S,A31V yielding just over 850 mg/L sfGFP.