RS/tRNA Foundational Publication Support
Wang, Lei, Zhiwen Zhang, Ansgar Brock, and Peter G Schultz. (2003) 2003. “Addition Of The Keto Functional Group To The Genetic Code Of Escherichia Coli.”. Proceedings Of The National Academy Of Sciences Of The United States Of America 100 (1): 56-61.
RS/tRNA Pair Development Year
2002
ncAA(s) Incorporated
p-acetyl-L-phenylalanine
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Site-directed spin labeling, electron paramagnetic resonance
RS Organism of Origin
Parent RS
RS Mutations
Y32L
D158G
I159C
L162G
D158G
I159C
L162G
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
Other tRNA Mutations
C17A
U17aG
U20C
G37A
U47A
U17aG
U20C
G37A
U47A
RS/tRNA Availability
n/a
Used in what cell line?
RS/tRNA Additional Notes
Using 1 mM pAcF, yielded 3.6 mg/L protein A(7) (~40% efficiency). Shown pure by MS, and able to be modified by fluoroscein and biotin hydrazides.
Two additional RSs were selected and not characterized. These were LW5 (Y32L, D158G, I159T, L162R) and LW6 (Y32A, D158G, I159G, A167I)
Two additional RSs were selected and not characterized. These were LW5 (Y32L, D158G, I159T, L162R) and LW6 (Y32A, D158G, I159G, A167I)