RS/tRNA Foundational Publication Support
Jang, Hyo Sang, Subhashis Jana, Robert J Blizzard, Joseph C Meeuwsen, and Ryan A Mehl. (2020) 2020. “Access To Faster Eukaryotic Cell Labeling With Encoded Tetrazine Amino Acids.”. Journal Of The American Chemical Society 142 (16): 7245-7249. doi:10.1021/jacs.9b11520.
RS/tRNA Usage Publications
Koh, Duk-Su, Anastasiia Stratiievska, Subhashis Jana, Shauna C Otto, Teresa M Swanson, Anthony Nhim, Sara Carlson, et al. (2024) 2024. “Genetic Code Expansion, Click Chemistry, And Light-Activated Pi3K Reveal Details Of Membrane Protein Trafficking Downstream Of Receptor Tyrosine Kinases.”. Biorxiv : The Preprint Server For Biology. doi:10.1101/2023.08.29.555449.
RS/tRNA Pair Development Year
2020
ncAA(s) Incorporated
Tet3-butyl
ncAA Structure (png, jpg, jpeg)
ncAA Utility
An amino acid that contains a 1,2,4,5-tetrazine-butyl group that can be used for bioorthogonal, click-chemistry reactions (bioconjugation) when reacted with strained trans-cyclooctenes (which can themselves be bound to various moieties such as PEG linkers, fluorophores, spin labels, etc.) either in vivo for eukaryotic systems or in vitro.
RS Organism of Origin
Parent RS
RS Mutations
L270G
N311G
C313A
N311G
C313A
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
The bacterial-compatible plasmid that contains the Tet3 RS (pDule-Tet3.0) is available on Addgene: Plasmid #174080.
Tet3-butyl can be purchased at the GCE4All site:
https://gce4all.oregonstate.edu/tetrazine-amino-acids
Tet3-butyl can be purchased at the GCE4All site:
https://gce4all.oregonstate.edu/tetrazine-amino-acids
Used in what cell line?