GCE Publications

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2023 Reprogramming Initiator and Nonsense Codons to Simultaneously Install Three Distinct Noncanonical Amino Acids into Proteins in E. coli. Han-Kai Jiang, Jeffery Tharp
2023 Bioorthogonal Tethering Enhances Drug Fragment Affinity for G Protein-Coupled Receptors in Live Cells. Jordan Mattheisen, Chris Limberakis, Roger Ruggeri, Matthew Dowling, Christopher Ende, Emilie Ceraudo, Thomas Huber, Christopher McClendon, Thomas Sakmar
2023 Linking chromatin acylation mark-defined proteome and genome in living cells. Fangfei Qin, Boyuan Li, Hui Wang, Sihui Ma, Jiaofeng Li, Shanglin Liu, Linghao Kong, Huangtao Zheng, Rongfeng Zhu, Yu Han, Mingdong Yang, Kai Li, Xiong Ji, Peng Chen
2023 Synthetic Organelles for Multiple mRNA Selective Genetic Code Expansions in Eukaryotes. Christopher Reinkemeier, Edward Lemke
2023 System-wide optimization of an orthogonal translation system with enhanced biological tolerance. Kyle Mohler, Jack Moen, Svetlana Rogulina, Jesse Rinehart
2023 7SK methylation by METTL3 promotes transcriptional activity Marcelo Perez-Pepe, Anthony Desotell, Hengyi Li, Wenxue Li, Bing Han, Qishan Lin, Daryl Klein, Yansheng Liu, Hani Goodarzi, Claudio Alarcón
2023 A New PETase from the Human Saliva Metagenome and Its Functional Modification via Genetic Code Expansion in Bacteria. Mirren White, Stephen Wallace
2023 Non-Canonical Amino Acids in Analyses of Protease Structure and Function. Peter Goettig, Nikolaj Koch, Nediljko Budisa
2023 Establishing the fundamental rules for genetic code expansion. Souvik Sinha, Mohd Ahsan, Giulia Palermo
2023 Protein Expression with Biosynthesized Noncanonical Amino Acids. Yong Wang, Wenkang Cai, Boyang Han, Tao Liu
2023 Engineering mutually orthogonal PylRS/tRNA pairs for dual encoding of functional histidine analogues. Christopher Taylor, Florence Hardy, Ashleigh Burke, Riley Bednar, Ryan Mehl, Anthony Green, Sarah Lovelock
2023 Capturing Covalent Catalytic Intermediates by Enzyme Mutants: Recent Advances in Methodologies and Applications. Yongchao Wang, Shan Tang
2023 Synthesis, Biochemical Characterization, and Genetic Encoding of a 1,2,4-Triazole Amino Acid as an Acetyllysine Mimic for Bromodomains of the BET Family. Sören Kirchgäßner, Michael Braun, Natascha Bartlick, Cengiz Koç, Christopher Reinkemeier, Edward Lemke, Thilo Stehle, Dirk Schwarzer
2023 Engineering enzyme activity using an expanded amino acid alphabet. Zachary Birch-Price, Christopher Taylor, Mary Ortmayer, Anthony Green
2023 The Expanded Central Dogma: Genome Resynthesis, Orthogonal Biosystems, Synthetic Genetics. Karola Gerecht, Niklas Freund, Wei Liu, Yang Liu, Maximilian Fürst, Philipp Holliger
2023 Genetic Code Expansion for Site-Specific Labeling of Antibodies with Radioisotopes. Christine Koehler, Paul Sauter, Benedikt Klasen, Christopher Waldmann, Stefanie Pektor, Nicole Bausbacher, Edward Lemke, Matthias Miederer
2023 Design of Polarity-Dependent Immunosensors Based on the Structural Analysis of Engineered Antibodies Islam J, Conroy P, Fercher C, Kim M, Yaari Z, Jones M, Bell Tdm, Caradoc-Davies T, Law R, Whisstock J, Heller D, Mahler S, Corrie S
2023 Bioconjugation of a Fibroblast Activation Protein Targeted Interleukin-4. Matthias Ruopp, Sha Zhu, Rafael Worschech, Dorothee Haas, Simone Maschauer, Olaf Prante, Lorenz Meinel, Tessa Lühmann
2023 An unnatural enzyme with endonuclease activity towards small non-coding RNAs. Noreen Ahmed, Nadine Ahmed, Didier Bilodeau, John Pezacki
2023 Creating Selenocysteine-Specific Reporters Using Inteins. Christina Chung, Dieter Söll, Natalie Krahn
2023 Crystal Structure of Pyrrolysyl-tRNA Synthetase from a Methanogenic Archaeon ISO4-G1 and Its Structure-Based Engineering for Highly-Productive Cell-Free Genetic Code Expansion with Non-Canonical Amino Acids. Tatsuo Yanagisawa, Eiko Seki, Hiroaki Tanabe, Yoshifumi Fujii, Kensaku Sakamoto, Shigeyuki Yokoyama
2023 Co-translational Installation of Posttranslational Modifications by Non-canonical Amino Acid Mutagenesis. Wei Niu, Jiantao Guo
2023 High-yield recombinant bacterial expression of C-, N-labeled, serine-16 phosphorylated, murine amelogenin using a modified third generation genetic code expansion protocol. Garry Buchko, Mowei Zhou, Cat Vesely, Jinhui Tao, Wendy Shaw, Ryan Mehl, Richard Cooley
2023 On the origin of the genetic code. Masayuki Seki
2023 Functional analysis of protein post-translational modifications using genetic codon expansion. Tao Peng, Tandrila Das, Ke Ding, Howard Hang