GCE Publications

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2023 Autonomous Synthesis of Functional, Permanently Phosphorylated Proteins for Defining the Interactome of Monomeric 14-3-3ζ. Phillip Zhu, Stanislau Stanisheuski, Rachel Franklin, Amber Vogel, Cat Vesely, Patrick Reardon, Nikolai Sluchanko, Joseph Beckman, Andrew Karplus, Ryan Mehl, Richard Cooley
2023 Optimized genetic code expansion technology for time-dependent induction of adhesion GPCR-ligand engagement. Marcel Streit, Mareike Hemberger, Stephanie Häfner, Felix Knote, Tobias Langenhan, Gerti Beliu
2023 Enabling Genetic Code Expansion and Peptide Macrocyclization in mRNA Display via a Promiscuous Orthogonal Aminoacyl-tRNA Synthetase. Sabrina Iskandar, Jarrett Pelton, Elizaveta Wick, Derek Bolhuis, Albert Baldwin, Michael Emanuele, Nicholas Brown, Albert Bowers
2023 Genetic Code Expansion in Mammalian Cells Through Quadruplet Codon Decoding. Yan Chen, Tianyu Gao, Xinyuan He, Wei Niu, Jiantao Guo
2023 FRETing about the details: Case studies in the use of a genetically encoded fluorescent amino acid for distance-dependent energy transfer. Michael Cory, Chloe Jones, Kyle Shaffer, Yarra Venkatesh, Sam Giannakoulias, Ryann Perez, Marshall Lougee, Eshe Hummingbird, Vinayak Pagar V, Christina Hurley, Allen Li, Robert Mach, Rahul Kohli, James Petersson
2022 Rational Design for High Bioorthogonal Fluorogenicity of Tetrazine-Encoded Green Fluorescent Proteins. Longteng Tang, Riley Bednar, Nikita Rozanov, Marcus Hemshorn, Ryan Mehl, Chong Fang
2022 Capture and mass spectrometry analysis of effector-substrate complexes using genetically incorporated photo-crosslinkers in host cells. Pan Li, Jingxiang Li, Haiyan Ren
2022 Generating Efficient Pyrrolysyl-tRNA Synthetases for Structurally Diverse Non-Canonical Amino Acids. Savanna Avila-Crump, Marcus Hemshorn, Chloe Jones, Lea Mbengi, Kyle Meyer, Joshua Griffis, Subhashis Jana, Grace Petrina, Vinayak Pagar V, Andrew Karplus, James Petersson, John Perona, Ryan Mehl, Richard Cooley
2022 Genetic Code Expansion and Bio-Orthogonal Labeling Reveal Intact HIV-1 Capsids inside the Nucleus. Leo James
2022 Generation of Photocaged Nanobodies for Intracellular Applications in an Animal Using Genetic Code Expansion and Computationally Guided Protein Engineering. Jack O'Shea, Angeliki Goutou, Jack Brydon, Cyrus Sethna, Christopher Wood, Sebastian Greiss
2022 The tRNA discriminator base defines the mutual orthogonality of two distinct pyrrolysyl-tRNA synthetase/tRNAPyl pairs in the same organism. Haolin Zhang, Xuemei Gong, Qianqian Zhao, Takahito Mukai, Oscar Vargas-Rodriguez, Huiming Zhang, Yuxing Zhang, Paul Wassel, Kazuaki Amikura, Julie Maupin-Furlow, Yan Ren, Xun Xu, Yuri Wolf, Kira Makarova, Eugene Koonin V, Yue Shen, Dieter Söll, Xian Fu
2022 Uncover New Reactivity of Genetically Encoded Alkyl Bromide Non-Canonical Amino Acids. Xin Shu, Sana Asghar, Fan Yang, Shang-Tong Li, Haifan Wu, Bing Yang
2022 Systematic Variation of Both the Aromatic Cage and Dialkyllysine via GCE-SAR Reveal Mechanistic Insights in CBX5 Reader Protein Binding. Kelsey Kean, Stefanie Baril, Kelsey Lamb, Sarah Dishman, Joseph Treacy, Kendall Houk, Eric Brustad, Lindsey James, Marcey Waters
2022 Recent Advances in Fluorescence Imaging by Genetically Encoded Non-canonical Amino Acids. Sanghee Lee, Jonghoon Kim, Minseob Koh
2022 Genetic Code Expansion: Another Solution to Codon Assignments. Kensaku Sakamoto
2022 Site-specific Incorporation of Phosphoserine into Recombinant Proteins in . Phillip Zhu, Ryan Mehl, Richard Cooley
2022 Expanding the chemical diversity of M13 bacteriophage. Grace Allen, Ashley Grahn, Katerina Kourentzi, Richard Willson, Sean Waldrop, Jiantao Guo, Brian Kay
2022 Tuning the Properties of Protein-Based Polymers Using High-Performance Orthogonal Translation Systems for the Incorporation of Aromatic Non-Canonical Amino Acids. Osher Gueta, Ortal Sheinenzon, Rotem Azulay, Hadas Shalit, Daniela Strugach, Dagan Hadar, Sigal Gelkop, Anat Milo, Miriam Amiram
2022 Expanding the functionality of proteins with genetically encoded dibenzo[,][1,4,5]thiadiazepine: a photo-transducer for photo-click decoration. Qin Xiong, Tingting Zheng, Xin Shen, Baolin Li, Jielin Fu, Xiaohu Zhao, Chunxia Wang, Zhipeng Yu
2022 Directed Evolution Pipeline for the Improvement of Orthogonal Translation Machinery for Genetic Code Expansion at Sense Codons. Wil Biddle, David Schwark, Margaret Schmitt, John Fisk
2022 Reprogramming the Biosynthesis of Precursor Peptide to Create a Selenazole-Containing Nosiheptide Analogue. Yingzi Tan, Miao Wang, Yijun Chen
2022 Measuring Repeat-Associated Non-AUG (RAN) Translation. Shaopeng Wang, Shuying Sun
2022 A multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics. Brandon Gassaway, Jiaming Li, Ramin Rad, Julian Mintseris, Kyle Mohler, Tyler Levy, Mike Aguiar, Sean Beausoleil, Joao Paulo, Jesse Rinehart, Edward Huttlin, Steven Gygi
2022 Engineered bacterial host for genetic encoding of physiologically stable protein nitration. Nikolaj Koch, Tobias Baumann, Jessica Nickling, Anna Dziegielewski, Nediljko Budisa
2022 Cryptochrome 1 as a state variable of the circadian clockwork of the suprachiasmatic nucleus: Evidence from translational switching. David McManus, Lenka Polidarova, Nicola Smyllie, Andrew Patton, Johanna Chesham, Elizabeth Maywood, Jason Chin, Michael Hastings