ncAA Database

Displaying 26 - 40 of 40 results
ncAA Name ncAA Structure ncAA Utility Moderation State
O-tert-butyl-L-tyrosine O-tert-butyl-L-tyrosine n/a Published: Under Review
p-azido-L-phenylalanine (pAzF) Image showing the ChemDraw Structure of p-azido-l-phenylalanine (pAzF) Used as a photocrosslinker, allowing for crosslinking and bioorthogonal ligation of protein. Published: Under Review
p-benzoyl-l-phenylalanine Image showing the ChemDraw Structure of p-benzoyl-l-phenylalanine Used as a photocrosslinker, allowing for crosslinking and bioorthogonal ligation of protein. Published: Under Review
p-Benzoyl-L-phenylalanine p-Benzoyl-L-phenylalanine N/A Published: Under Review
p-Boronophenylalanine p-Boronophenylalanine Used for protein purification, diol conjugation, Suzuki Coupling and as latent tyrosine residue Published: Under Review
p-Methoxy-L-phenylalanine Image showing the Structure of p-Methoxy-L-phenylalanine First UAA
incorporation by
amber suppression 		Published: Under Review
phosphoserine pSer Used to produce proteins site-specifically phosphorylated at serine residues, typically at positions where phosphoserine naturally occurs as a post-translational modification Published: Fully Reviewed
phosphothreonine pThr Used to produce site-specifically phosphorylated proteins at a threonine residue via genetic code expansion. Published: Fully Reviewed
phosphotyrosine pTyr Used to produce site-specifically phosphorylated proteins at a tyrosine residue via genetic code expansion versus other protein phosphorylation strategies. Published: Under Review
pNO2ZLys para-nitro-ZLys Photo-­cross-linking agent, but no tests were done on this ncAA in the founding paper. Crosslinking experiments were only done with TmdZLys Published: Fully Reviewed
propargylglycine Propargylglycine copper-catalyzed alkyne-azide click chemistry (CuACC) can be used for time-resolved, cell-selective proteomic analyses Published: Fully Reviewed
SCO-L-Lysine SCO-L-Lysine Click chemistry reactions with azides (SPAAC - strain-promoted azide–alkyne cycloaddition) and tetrazines (SPIEDAC - strain promoted inverse electron demand Diles-Alder cycloaddition) Published: Fully Reviewed
Tet2-Et Tet2-Et An amino acid that contains a 1,2,4,5-tetrazine-ethyl group that can be used for bioorthogonal, click-chemistry reactions (bioconjugation) when reacted with trans-cyclooctenes (TCOs) which can themselves be bound to various moieties such as PEG linkers, fluorophores, spin labels, etc.. These click reactions require no catalysts and can be carried out either in vivo or in vitro. Also, when reacted with strained TCOs (sTCOs), the reaction rates are extremely fast, achieving second order rate constants in excess of 10,000 per molar per second. Published: Under Review
Tet3-butyl Tet3-butyl An amino acid that contains a 1,2,4,5-tetrazine-butyl group that can be used for bioorthogonal, click-chemistry reactions (bioconjugation) when reacted with strained trans-cyclooctenes (which can themselves be bound to various moieties such as PEG linkers, fluorophores, spin labels, etc.) either in vivo for eukaryotic systems or in vitro. Published: Under Review
TmdZLys TmdZLys Photo-­cross-linking agent, and head to head comparison with Bpa and TmdPhe showed that TmdZLys led to successful crosslinking from more positions including those further apart, up to 15 Å. Tests were done with the GRB2 protein crosslinking with both the EGF receptor and the SHC receptor protein. Published: Fully Reviewed