Nε-Allyloxycarbonyl-I-lysine(AlocLys) RS 1

RS/tRNA Foundational Publication Support

Yanagisawa, Tatsuo, Ryohei Ishii, Ryuya Fukunaga, Takatsugu Kobayashi, Kensaku Sakamoto, and Shigeyuki Yokoyama. (2008) 2008. “Multistep Engineering of Pyrrolysyl-TRNA Synthetase to Genetically Encode N(epsilon)-(o-Azidobenzyloxycarbonyl) Lysine for Site-Specific Protein Modification”. Chemistry & Biology 15 (11): 1187-97. doi:10.1016/j.chembiol.2008.10.004.

RS/tRNA Usage Publications

Yanagisawa, Tatsuo, Ryohei Ishii, Ryuya Fukunaga, Takatsugu Kobayashi, Kensaku Sakamoto, and Shigeyuki Yokoyama. (2008) 2008. “Multistep Engineering of Pyrrolysyl-TRNA Synthetase to Genetically Encode N(epsilon)-(o-Azidobenzyloxycarbonyl) Lysine for Site-Specific Protein Modification”. Chemistry & Biology 15 (11): 1187-97. doi:10.1016/j.chembiol.2008.10.004.

RS/tRNA Pair Development Year

2008

ncAA(s) Incorporated

RS Organism of Origin

Methanosarcina mazei

Parent RS

Pyl RS

RS Mutations

Y384F

tRNA Organism of Origin

Methanosarcina mazei

Parent tRNA

Pyl tRNA

tRNA Anticodon

CUA

RS/tRNA Availability

N/A

Used in what cell line?

DH10B