MmPylRS(Y306A,Y384F) RS/tRNA Pair Development Year 2008 ncAA(s) Incorporated Nε-(o Azidobenzyloxycarbon yl)-L-lysine (AzZLys) N6-(Benzyloxycarbonyl)-L-lysine(ZLys) 4'-TCOK (TCO) endo-BCN-L-Lysine exoBCN-Lysine 2'-axialTCOK (TCO*) TmdZLys pNO2ZLys N6-[2-(furan-2-yl)ethoxy]carbonyl Lys 2,2,5,5-tetramethyl-pyrrolin-1-oxyl
PylRS(N346A/C348A) RS/tRNA Pair Development Year 2012 ncAA(s) Incorporated 2-iodo-L-phenylalanine 2-chloro-L-phenylalanine 2-bromo-L-phenylalanine 2-methoxy-L-phenylalanine 2-Cyano-L-phenylalanine O-allyl-L-tyrosine O-Methyl-L-tyrosine O-Benzyl-L-tyrosine O-allyl-L-tyrosine O-tert-butyl-L-tyrosine O-propargyl-L-tyrosine O-3-butenyl-L-tyrosine O-4-pentenyl-L-tyrosine 3-chloro-L-phenylalanine 3-bromo-L-phenylalanine 3-iodo-L-phenylalanine 3-methyl-L-phenylalanine 3-methoxy-L-phenylalanine 3-trifluoromethyl-L-phenylalanine 3-cyano-L-phenylalanine 3-nitro-L-phenylalanine 3-acetyl-L-phenylalanine 3-allyl-L-phenylalanine 3-azido-L-phenylalanine