ncAA Structure (png, jpg, jpeg)
ncAA Foundational Publication Support
Young, Douglas D, Travis S Young, Michael Jahnz, Insha Ahmad, Glen Spraggon, and Peter G Schultz. (2011) 2011. “An Evolved Aminoacyl-Trna Synthetase With Atypical Polysubstrate Specificity.”. Biochemistry 50 (11): 1894-900. doi:10.1021/bi101929e.
Wang, Yane-Shih, Xinqiang Fang, Ashley L Wallace, Bo Wu, and Wenshe R Liu. (2012) 2012. “A Rationally Designed Pyrrolysyl-Trna Synthetase Mutant With A Broad Substrate Spectrum.”. Journal Of The American Chemical Society 134 (6): 2950-3. doi:10.1021/ja211972x.
ncAA Usage Publications
Chen, Wan-Na, Kekini Kuppan, Michael Lee, Kristaps Jaudzems, Thomas Huber, and Gottfried Otting. (2015) 2015. “O-Tert-Butyltyrosine, An Nmr Tag For High-Molecular-Weight Systems And Measurements Of Submicromolar Ligand Binding Affinities.”. Journal Of The American Chemical Society 137 (13): 4581-6. doi:10.1021/jacs.5b01918.
Loh, Choy Theng, Luke A Adams, Bim Graham, and Gottfried Otting. (2018) 2018. “Genetically Encoded Amino Acids With Tert-Butyl And Trimethylsilyl Groups For Site-Selective Studies Of Proteins By Nmr Spectroscopy.”. Journal Of Biomolecular Nmr 71 (4): 287-293. doi:10.1007/s10858-017-0157-y.
Jabar, Shereen, Luke A Adams, Yao Wang, Luigi Aurelio, Bim Graham, and Gottfried Otting. (2017) 2017. “Chemical Tagging With Tert-Butyl And Trimethylsilyl Groups For Measuring Intermolecular Nuclear Overhauser Effects In A Large Protein-Ligand Complex.”. Chemistry (Weinheim An Der Bergstrasse, Germany) 23 (53): 13033-13036. doi:10.1002/chem.201703531.
ncAA Utility
NMR tag for proteins
ncAA Source
Exogenous - Purchased
ncAA Availability
Can purchased from https://www.sigmaaldrich.com/US/en/product/aldrich/533130
RS/tRNA Pair Usage Information
incorporated at low levels (2 mg/L) with fidelity in E coli grown with 2 mM ncAA in GMML media. NMR studies were done using the pCNF-RS1 synthetase.
ncAA Synonyms
Tby
H-Tyr(tBu)-OH
O-tert-butyl-L-tyrosine
L-Tyrosine, O-(1,1-dimethylethyl)-
(S)-2-Amino-3-(4-(tert-butoxy)phenyl)propanoic acid
H-L-Tyr(tBu)-OH
(2S)-2-amino-3-[4-[(2-methylpropan-2-yl)oxy]phenyl]propanoic acid
(2S)-2-amino-3-[4-(tert-butoxy)phenyl]propanoic acid
H-Tyr(tBu)-OH
O-tert-butyl-L-tyrosine
L-Tyrosine, O-(1,1-dimethylethyl)-
(S)-2-Amino-3-(4-(tert-butoxy)phenyl)propanoic acid
H-L-Tyr(tBu)-OH
(2S)-2-amino-3-[4-[(2-methylpropan-2-yl)oxy]phenyl]propanoic acid
(2S)-2-amino-3-[4-(tert-butoxy)phenyl]propanoic acid
ChEBI ID
233946