MbPylRS-WT RS/tRNA Pair Development Year 2006 ncAA(s) Incorporated Norbornene-O–L-Lysine (NBO) N6-((2-azidoethoxy)carbonyl)-L-lysine Nε-(tert-Butoxycarbonyl)-L-lysine (BocLys) Nε -D-Prolyl-L-lysine N6-[(2 Propynyloxy)carbonyl]-L-lysine
PylRS(N346A/C348A) RS/tRNA Pair Development Year 2012 ncAA(s) Incorporated 2-iodo-L-phenylalanine 2-chloro-L-phenylalanine 2-bromo-L-phenylalanine 2-methoxy-L-phenylalanine 2-Cyano-L-phenylalanine O-allyl-L-tyrosine O-methyl-L-tyrosine O-Benzyl-L-tyrosine O-allyl-L-tyrosine O-tert-butyl-L-tyrosine (Tby) O-propargyl-L-tyrosine O-3-butenyl-L-tyrosine O-4-pentenyl-L-tyrosine 3-chloro-L-phenylalanine 3-bromo-L-phenylalanine 3-iodo-L-phenylalanine 3-methyl-L-phenylalanine 3-methoxy-L-phenylalanine 3-trifluoromethyl-L-phenylalanine 3-cyano-L-phenylalanine 3-nitro-L-phenylalanine 3-acetyl-L-phenylalanine 3-allyl-L-phenylalanine 3-azido-L-phenylalanine 3-Formyl-L phenylalanine
TFAcKRS (Mb) RS/tRNA Pair Development Year 2018 ncAA(s) Incorporated N6-Trifluoroacetyl-L-lysine Nε-acetyl-L-lysine
