RS/tRNA Foundational Publication Support
Yanagisawa, Tatsuo, Nobumasa Hino, Fumie Iraha, Takahito Mukai, Kensaku Sakamoto, and Shigeyuki Yokoyama. (2012) 2012. “Wide-Range Protein Photo-Crosslinking Achieved By A Genetically Encoded N(Ε)-(Benzyloxycarbonyl)Lysine Derivative With A Diazirinyl Moiety.”. Molecular Biosystems 8 (4): 1131-5. doi:10.1039/c2mb05321g.
RS/tRNA Pair Development Year
2012
ncAA(s) Incorporated
pNO2ZLys
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Photo-cross-linking agent, but no tests were done on this ncAA in the founding paper. Crosslinking experiments were only done with TmdZLys
TmdZLys
ncAA Structure (png, jpg, jpeg)
ncAA Utility
Photo-cross-linking agent, and head to head comparison with Bpa and TmdPhe showed that TmdZLys led to successful crosslinking from more positions including those further apart, up to 15 Å. Tests were done with the GRB2 protein crosslinking with both the EGF receptor and the SHC receptor protein.
RS Organism of Origin
Parent RS
RS Mutations
Y306G
Y384F
Y384F
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
CUA
RS/tRNA Availability
N/A
Used in what cell line?
RS/tRNA Additional Notes
This RS was discovered in a screen, but ended up not being used because experiments showed that the MmPylRS(Y306A,Y384F) worked better for both of the ncAAs tested