RS/tRNA Foundational Publication Support
Mukai, Takahito, Takatsugu Kobayashi, Nobumasa Hino, Tatsuo Yanagisawa, Kensaku Sakamoto, and Shigeyuki Yokoyama. (2008) 2008. “Adding L-Lysine Derivatives To The Genetic Code Of Mammalian Cells With Engineered Pyrrolysyl-Trna Synthetases.”. Biochemical And Biophysical Research Communications 371 (4): 818-22. doi:10.1016/j.bbrc.2008.04.164.
RS/tRNA Pair Development Year
2008
ncAA(s) Incorporated
N6-(Benzyloxycarbonyl)-L-lysine(ZLys)
ncAA Structure (png, jpg, jpeg)
ncAA Utility
n/a
RS Organism of Origin
Parent RS
RS Mutations
R61K
G131E
L309A
C348V
Y384F
G131E
L309A
C348V
Y384F
tRNA Organism of Origin
Parent tRNA
tRNA Anticodon
cUA
RS/tRNA Availability
N/A
RS/tRNA Additional Notes
ZLysRS and GRB2(Am111)-FLAG expressed in HEK293 c-18 cells with 1mM Z-lysine present in growth medium, full-length product detected by Western blotting. No product was detected in the absence of Z-lysine.
ZLysRS-tRNA^Pyl system was used to produce full-length GST(Am25) in E. coli cells at 6.9mg/l in a medium containing 1mM Z-lysine, system did not produce product in the absence of Z-lysine, confirmed by mass spectrometry.
ZLysRS exhibited efficient amber suppression in yeast cells, evidenced by B-Gal activity in MaV203
ZLysRS-tRNA^Pyl system was used to produce full-length GST(Am25) in E. coli cells at 6.9mg/l in a medium containing 1mM Z-lysine, system did not produce product in the absence of Z-lysine, confirmed by mass spectrometry.
ZLysRS exhibited efficient amber suppression in yeast cells, evidenced by B-Gal activity in MaV203