Pyl tRNA

G1 PylT/RS(Y125A)

RS/tRNA Pair Development Year
2020
ncAA(s) Incorporated
N6-(1-Methylcycloprop-2-enecarboxamido)lysine (CpK)
exoBCN-L-Lysine
2'-axialTCOK (TCO*)
N6-((2-azidoethoxy)carbonyl)-L-lysine(AzeoK)
propargylglycine

AznLRS

RS/tRNA Pair Development Year
2018
ncAA(s) Incorporated
Azidonorleucine

PylRS-AS

RS/tRNA Pair Development Year
2017
ncAA(s) Incorporated
Pentafluoro-D-phenylalanine
2,3,4,5-tetrafluorophenylalanine
3,4,5-trifluorophenylalanine
3,5-difluorophenylalanine

Mx1201PylRS

RS/tRNA Pair Development Year
2018
ncAA(s) Incorporated
4'-TCOK (TCO)
NĪµ-acetyl-L-lysine

NESPylRS

RS/tRNA Pair Development Year
2017
ncAA(s) Incorporated
Boc-LysOH
N6-(Benzyloxycarbonyl)-L-lysine (Z-Lys)
p-azido-L-phenylalanine (pAzF)

MmAcK3RS(IPYE)

RS/tRNA Pair Development Year
2017
ncAA(s) Incorporated
NĪµ-acetyl-L-lysine

MbAcK3RS(IPYE)

RS/tRNA Pair Development Year
2017
ncAA(s) Incorporated
NĪµ-acetyl-L-lysine

MbPylRS(IPYE)

RS/tRNA Pair Development Year
2017
ncAA(s) Incorporated
NĪµ-(tert-Butoxycarbonyl)-L-lysine (BocLys)

PylHRS

RS/tRNA Pair Development Year
2014
ncAA(s) Incorporated
3-methyl-DL-histidine
beta-(3-Pyridyl)-DL-alanine
3-(2-furyl)alanine
3-(2-Thienyl)alanine
D-2-(5-BromoThienyl)-alanine

MmPylRS(IPYE)

RS/tRNA Pair Development Year
2017
ncAA(s) Incorporated
NĪµ-(tert-Butoxycarbonyl)-L-lysine (BocLys)

chPylRS(IPYE)

RS/tRNA Pair Development Year
2017
ncAA(s) Incorporated
NĪµ-(tert-Butoxycarbonyl)-L-lysine (BocLys)

Kcr-RS

RS/tRNA Pair Development Year
2012
ncAA(s) Incorporated
NĪµ-Crotonyl-L-lysine

AcrKRS

RS/tRNA Pair Development Year
2014
ncAA(s) Incorporated
NĪµ-Acryloyl-L-lysine

D9-nitroTyr-MaPylRS

RS/tRNA Pair Development Year
2022
ncAA(s) Incorporated
3-nitro-L-tyrosine
3-iodo-L-tyrosine

F4-nitroTyr-MbPylRS

RS/tRNA Pair Development Year
2019
ncAA(s) Incorporated
3-nitro-L-phenylalanine
3-nitro-L-tyrosine

MmPylRS-WT

RS/tRNA Pair Development Year
2009
ncAA(s) Incorporated
Pyrrolysine (Pyl)
l-Cys-e-Lys
D-Cys-Īµ-Lys (ā€œ(S,S)ā€)
NĪµ-(Spiro[2.3]hex-1 ene-5 methoxycarbonyl)-L lysine
NĪµ-Crotonyl-L-lysine
NĪµ-Butyryl-L-lysine
NĪµ-Propionyl-L-lysine
N6-((2-azidoethoxy)carbonyl)-L-lysine(AzeoK)
N6-(1-Methylcycloprop-2-enecarboxamido)lysine (CpK)

NBK-1

RS/tRNA Pair Development Year
2010
ncAA(s) Incorporated
o-Nitrobenzyl oxycarbonyl-NĪµ-L lysine

ThzKRS

RS/tRNA Pair Development Year
2011
ncAA(s) Incorporated
Ne-L-Thiapropyl-L-lysine

CysKRS

RS/tRNA Pair Development Year
2011
ncAA(s) Incorporated
l-Cys-e-Lys
D-Cys-Īµ-Lys (ā€œ(S,S)ā€)

norb-PylRS

RS/tRNA Pair Development Year
2012
ncAA(s) Incorporated
Norbornene-methoxycarbonyl-Lysine

DZKRS

RS/tRNA Pair Development Year
2011
ncAA(s) Incorporated
(3-(3-Methyl-3H diazirine-3-yl)-propaminocarbonyl- NĪµ-L-lysine ( DiZPK)
DiZHSeC

PCKRS

RS/tRNA Pair Development Year
2010
ncAA(s) Incorporated
N-epsilon-((1-(6-nitrobenzo[d][1,3]dioxol-5-yl)ethoxy)carbonyl)-L-lysine

ACPK-RS

RS/tRNA Pair Development Year
2011
ncAA(s) Incorporated
N6-((2-azidoethoxy)carbonyl)-L-lysine(AzeoK)
N6-(((1R,2R)-2-azidocyclopentyloxy)carbonyl)-L-lysine (ACPK)
BlocLys
PlocLys
N6-((Pent-4-yn-1-yloxy)carbonyl)-L-lysine

ZLysRS

RS/tRNA Pair Development Year
2008
ncAA(s) Incorporated
N6-(Benzyloxycarbonyl)-L-lysine (Z-Lys)
Pyl tRNA